ࡱ> N 6 !"#$%&'()*+,-./012345F789:;<=>?@ABCDEGHIJKLMOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|}~Root Entry F+ SummaryInformation(@DocumentSummaryInformation8 WordDocument:  !"#$%&'()*+,- Oh+'00 < H T `lt|hQ Ty140-[Rir *sk|W O~ghKmel.doc4@@S/6O@f@do+$=WPS Office_11.1.0.10072_F1E327BC-269C-435d-A152-05C5408002CA ՜.+,D՜.+, X`ht   (\dlKSOProductBuildVer2052-11.1.0.100720TableData WpsCustomData xg< xg< g g F+ F+ :X:Xccq*q*Y,Y,6s=6s=DD_:sK_:sK*|U*|U]`]``bd`bd?}e?}elmlmmm+^+@`@CJOJPJo(56mH sH l^` o()^` o(.^` o(.^` o()^` o(.^` o(.^` o()^` o(.^`CJOJPJo(:yOH^H\`\o()^\`\o(.^\`\o(.4^4\`\o() ^ \`\o(.| ^| \`\o(. ^ \`\o()^\`\o(.^k`kCJOJPJo(aJ56:yO^k`k ko()^k`k ko(.^k`k ko(.^k`k ko()^k`k ko(.^k`k ko(.^k`k ko()^k`k ko(. ^ K`KCJOJPJo(56H* k^k` 9o(k^k` 9o(.k^k` 9o(.k^k` 9o()k^k` 9o(.k^k` 9o(.k^k` 9o()k^k` 9o(.+^+@`@CJOJPJo(aJ56H*l^` H*o()^` H*o(.^` H*o(.^` H*o()^` H*o(.^` H*o(.^` H*o()^` H*o(.^`CJOJPJQJo(aJ560T^`CJOJPJQJo(^JaJ56>*7S*4*H*<@EHKH89;XT\].0^`CJOJPJQJo(56..0^`CJOJPJQJo(56...0 ^`CJOJPJQJo(56 ....0 ^`CJOJPJQJo(56 .....0 ^`CJOJPJQJo(56 .....0 ^v`v o(....... E^E\`\ o(........o^oW`Wo(^`o(V.0P^P` Po(..^<`< b o(...  ^ ` so( ....  ^ ` o( ..... ^` o( ...... ^v`v o(....... ^\`\ o(........A^Ah`ho(  ^c`c B*phOJQJo(^b`b B*phOJQJo(\^\` o(.^` O o() ^ `  o(. ^ `  o(.< ^< `  o()t ^t ` /o(.^` o() ^ ` o() ^ ` o(. ^ ` 0o(. ^ ` Lo() ^ ` ho(. ^ ` o(. ^ ` o() ^ ` o(.G^G]`] HCJOJPJo(aJ56)^]`] o()^\`\ CJOJPJo(aJ56()3^3]`] 4o(. ^ ]`]  o(){ ^{ ]`] | o(. ^ ]`]  o(.^]`] o()g^g]`] ho(. ^k`kCJOJPJQJo(aJ56H*:yO^`H*o(^`H*o(2.2.^` H*o(.^` H*o()^` H*o(.^` H*o(.^` H*o()^` H*o(.^`CJOJPJQJo(56V0^`CJOJPJQJo(560^`CJOJPJQJo(56.0^`CJOJPJQJo(56..0^`CJOJPJQJo(56 ...0 ^`CJOJPJQJo(56 ....0 ^`CJOJPJQJo(56 .....0 ^v`v o(....... E^E\`\ o(........^W`W o(7^7`o(h.0^` o(..^<`< o(... N^N`  o( .... PKSKS:/.-8-.-8-f4fhfZLZD [xjklN\nXoo}`$]h]2Z@j)g+X-(A`L ICS  FORMTEXT 65.020.30  FORMTEXT B 44 DB23 ў_lw0WehQ DB 23/T  FORMTEXT XXXXX  FORMTEXT XXXX  FORMTEXT        FORMTEXT [Rir *sk|W O~ghKmel _Bla?z ;NwIUSMO -NVQNyf[bT\n}Q;Sxvz@b T | Nؚi_ T|5u݋15114516823 T|O{gaocaixia@caas.cn  FORMTEXT       FORMTEXT XXXX -  FORMTEXT XX -  FORMTEXT XXS^  FORMTEXT XXXX -  FORMTEXT XX -  FORMTEXT XX[e  FORMTEXT ў_lw^:Wvcw{t@\   S^ MR  ,ghQOnc GB/T 1.1-2009 vQĉRwI0 ,ghQ1uў_lwQNQQgScQ0 ,ghQ1uў_lw[RirNNhQSb/gYXTOR_S0 ,ghQwIUSMO-NVQNyf[bT\n}Q;Sxvz@b0uQN'Yf[0NSQN'Yf[0R\QN'Yf[0 ,ghQ;NwINؚi_0H*m\0uuV0R 0ߏo0 [Rir *sk|W O~ghKmel V ,ghQĉ[N[*sk|W O~ghKmelvSt07hTǑƖ07hTDNAvcS0_ir^R0PCRibX0pencYtT~gRg0 ,ghQ(uN[*sk|W O~gvhKmNRg0 ĉ'`_(ueN NReN[N,geNv^(u/f_ NS\v0Q/flegv_(ueN NlegvHr,g(uN,geN0Q/f Nlegv_(ueN vQgeHr,gSb@b gvO9eUS (uN,geN0 GB/T 35892 [Rir y)R&Ot[gcWS NY/T 541 Riruu[[hǑ7hel NY/T 1673 \uy_kSfDNAW OY7h'`hKmb/gĉ z St ~|SO DNA /f8hY/UNvW Oir( W O NwQ g;N'` HT%N\~~ cSWV~DNA TbN[yr_'`_ir[~|SO DNA D-Loop :SۏL PCR ibX 8hxQ5u  ^ ` o( ..... J ^J ` &o( ...... ^v`v 7o(....... E^E\`\ Io(........^`CJOJPJQJo(56h0^` o(.^` o(..^<`< o(...  ^ `  o( ....  ^ `  o( ..... ^` o( ...... *^*v`v *o(....... ^\`\ o(........!^`CJOJPJQJo(56RHd@D0U_0(^`CJOJPJQJo(56hRHd@KH.0^`CJOJPJQJo(56..0^`CJOJPJQJo(56...0 ^`CJOJPJQJo(56 ....0 ^`CJOJPJQJo(56 .....0 ^`CJOJPJQJo(56......0 *^*v`v *o(....... ^\`\ o(........G^G]`] GCJOJPJo(56)G^G]`] HCJOJPJo(56)^]`] o(.^]`] o(.3^3]`] 4o() ^ ]`]  o(.{ ^{ ]`] | o(. ^ ]`]  o()^]`] o(.^`CJOJPJQJo(56l^` to()^` to(.^` to(.^` to()^` to(.^` to(.^` to()^` to(.6s=lmc?}eg xg< _:sK]`Y,q*D:XmF+ *|U`bd&66666666 0@P`p6666 0@P`p 0@P`p 0@P`p 0@P`p 0@P`p 0@P`pJ@Jcke a$$1$ CJaJKHmH sH nHtH_H$A@$؞k=W[SONi@Nnfh*RHd@mHsHnHtH$*@!$>\l_(uH*2V@12]vc B* ph>*8OA8S^CJOJPJaJRHd@UEH@OQ@6k CharCJOJmH sH nHtH_HPOaPL:yO Char(CJOJQJaJKHmH sH nHtH_H*ORq*H DU_lQ_ CharRORG zh Char Char CJOJPJmH sH nHtH_HJ@J"}_ 5a$$^.`.CJOJQJaJ: @b:"}_ 1a$$ S$ OJaJF@FvU_ 5a$$WD,,`, $ OJaJ4@4vU_ 2  $ OJaJF@FvU_ 4a$$WD` $ OJaJ.+@.>\le,g a$$G$J @J"}_ 4a$$H^H.`.CJOJQJaJ@"@@l CJOJPJQJ^JaJJ@J"}_ 9!a$$b^b.`.CJOJQJaJF@FvU_ 6"a$$WD` $ OJaJF@FvU_ 8#a$$WDX_`_ $ OJaJF@FvU_ 7$a$$WD` $ OJaJJ@J"}_ 6%a$$^.`.CJOJQJaJJ@J"}_ 7&a$$^.`.CJOJQJaJF@FvU_ 3'a$$WDdd`d $ OJaJ8Y@8ech~gV(-D M 2@2u w )a$$G$CJaJP@Ple,g!* & F a$$G$  CJOJaJJ@J"}_ 8+a$$^.`.CJOJQJaJJ @J"}_ 2,a$$^.`.CJOJQJaJJ@JvU_ 1 -XDYDa$$ $ OJaJ:@:vU_ 9.a$$^CJaJ> @>u/a$$G$UDd]CJaJJ @J"}_ 30a$$v^v.`.CJOJQJaJH!@H"}_h1a$$xxOJQJaJ5\]fO"f DU_W[kSRyN~ 2 & F G GCJOJmH sH nHtH_H(Oq2(vQN[eeg30Ob0 N~agh 4 & F@&DObDDU_V~agh5 h & F@& h^Ob^k%6a$$8$7$WD` iR$ CJOJmH sH nHtH_HXOarXVhlf*7 iR$H^HWD\`\ iR$ CJaJzOzvQNhQy'8da$$&#$/.0$,CJ0OJPJQJaJ4@mH sH nHtH_H8O8~~~9&`#$/.DOQbDDU_ N~agh: h & F@& h8Ob8N~agh; & F@&22<O<vQNhQh_<&ARHBOB\bhQe Ty =dpr CJOJaJrObrMR0_h*>a$$$$@&-DM 0 CJ OJPJmH sH nHtH_HPOP:yOQ[?WD` CJOJaJmH sH nHtH_H:O:N~e@XDYDOJPJObDU_zh7A & FdXDddYDda$$9D7$@&4$5$ h$CJOJPJKHmH sH nHtH_HPOP:yOB & Fa$$1$ CJOJaJmH sH nHtH_H>O12> \bhQe?zOo` CdLCJ^OB^hQfN_GYpeuDa$$x] CJOJaJmH sH nHtH_HDOQbDDU_N~aghE h & F@& hzObz\bhQNfOo`/Fda$$9&m^ #+D#$/0$ CJOJaJmH sH nHtH_HbObbzh"G & F8XDd8YDda$$@& CJOJPJmH sH nHtH_H8Oab8DU_lQ_H iR$ iR$ fOf DU_peW[SRyN~ I & F H HCJOJmH sH nHtH_HfOfhQfN w_GYpeuJa$$ :r $CJOJPJaJmH sH nHtH_HdObdN~aghK & FXD2YD2@&$CJOJPJaJmH sH nHtH_HbObb:yOL & F` h(CJOJQJaJKHmH sH nHtH_H6O16 \bhQe?zOo`2MuNOqN:yON & FXDYD@& CJOJPJaJ^O^S^egO&7+D#$.0$CJPJmH sH nHtH_H0O0vQNS^eg P&hObhDU_hh7Q & F2XD22YD2a$$^`  OJPJaJfO"fRy%N~ !R & F a$$ HCJOJmH sH nHtH_H:O2:\bhQe?z{|+R SdCJ:OAB: N~eTXDYDOJPJ`OR`DU_VhS1U & F da$$1$$$@&^k`k B*phjObj Se.s0"}_h-Va$$1$$$@&-DM OJPJaJKH.Or.[eeg Wa$$&XOXhQfN_vPpeuXx^ CJOJaJmH sH nHtH_HhOh W[kSRyN~ Y & F Ha$$ HCJOJmH sH nHtH_H^O^SRy N~ Z & F  CJOJmH sH nHtH_H^Ob^DU_hhS/[ & F da$$@&+^+@`@ B*phtObtS^)\a$$&f&9+Dn#$/}.0$'CJOJ5RH@mH sH nHtH_HOhQh_@]da$$-DM &@z +Dm#$/.0$#CJ`aJ`5RHmH sH nHtH_H:O: \bN'` z^hƋ^OJPJJOQJDU_V~e_ hXDYD OJPJaJtOt\bhQ Ty.`dXa$$9D1$&%+D#$0$ CJ4OJPJmH sH nHtH_H^Ob^ DU_lQ_S6Rh&{a8$7$1$ iR$ OJaJKHmHsHnHtHJO"JDU_ N~eb hXDYD OJPJaJNOabN ckelQ_S6Rh&{"c iR$WD` iR$ >OB>vQNS^ dd; OJPJ5pObpDU_N~agh7e & F2XD22YD28$7$1$@&9D4$5$ hOJPJaJKHROabRDU_h(f iR$a$$WD` iR$ OJPJJOQrJDU_N~eg hXDYD OJPJaJ>O>\bckeha$$mH sH nHtH_HFOabF:yOTeW[i iR$h`h iR$ CJXOXlcke j & F a$$ CJOJaJmH sH nHtH_HhOh peW[SRyN~ k & F a$$ CJOJmH sH nHtH_H:OQ:N~elXDYDOJPJ:O:V~emXDYDOJPJhObhDU_Vh7n & F 2XD22YD2a$$^` k OJPJaJ(Ob(lcke o0OAb0V~agh p & F@&RORv!k0"}_lhKmvvag&^ [ibXNirۏLvcKm^ RgUS PWY7h^TW OY7h'`N nx[[*svk|W O~g0 7hTǑƖ Ǒ7h*NSO^wQY*sTyvxQWyr_ k*NTyǑƖBl NNQe@sQ|v*s*NSO N\N404Y0 cgqNY/T 541ǑƖ[*sv3b>\~~ Riry)Ru_ GB/T 3589207hTvǑƖSOX[^ncSDNAvBl0~U_Pge Ty0egn0|10ǑƖe00WpTOX[agN0 7hTDNAvcS 9hnc NY/T 1673 -NvZ/lNl bFUTSՋBRvcS7hT DNA +}YRIQIQ^Km[ DNA Sm^T~^ Sm^50 ng/L ~^OD260/OD280=1.6^1.80 _ir^R D-loop F 5'-CCA AGA CTC AAG GAA GGA GA-3'0 D-loop R 5'-GGC GCG GAT ACT TGC ATG TG-3'0 PCRibX PCRibXSO| PCR S^SO|:N 50 LTaqv (5 U/L) 0.25 L 10 Taq buffer (+T Mg2+) 5 L dNTP mix (2.5 mmol/L) 4 L N0 N8n_ir(10 pmol/L)T1 L DNA !jg 2.5 L (~ 350 ng/L) ddH2O 36.3 L0 Tezzz}v[gq0 PCRibX z^ ibX z^:N94! S'` 4 min94! S'` 30 s 60! kp 30 s 72! ^8O1 min 30!k_s72! ^8O 4 min0 PCRNirhKm S 5 L PCRNirN N7hQmmT N 1% v*>*o(o(o(o(o(o(o(mHsHnHtHmHsHnHtHo(mHsHnHtHmHsHnHtHmHsHnHtHo(mHsHnHtHmHsHnHtHo(mHsHnHtH*8^bdn {ywqF$If~8<ps$$If:V 644l44l0&5~&w$Ifww  J Z \ hOWDb`b$If&%+D#$0$WDb`b$If&%+D#$0$^=`~~s$$If:V 644l44l0 $5$  oS$If&%+D#$0$WDb`b$If&%+D#$0$WDb`b$If&%+D#$0$WDb`b$If&%+D#$0$WDb`b$If&%+D#$0$WDb`b$If&%+D#$0$ckeqda$$CJOJmH sH nHtH_HbObbVvl#ra$$1$VDH^HWD8\`\CJOJmH sH nHtH_HtObt v!k0hQ Tyh3sd4a$$1$$$@&-DM 0CJ OJPJaJKHXOBXlt & F a$$8$7$1$ CJOJaJmH sH nHtH_HJOQRJDU_N~eu hXDYD OJPJaJHObHRy% N~ v & F  OJaJfOrfe.sR{|Sw1$9D&@#$/.0$$CJOJPJaJmH sH nHtH_H\O\Ryf-xda$$9DH$VD^WD88`8 OJaJKHVObVly & Fa$$8$7$1$ CJOJaJmH sH nHtH_H^O^\bhQS1za$$9D8$7$1$5$3$4CJmH sH nHtH_HXOXRy  N~ { & F a$$1$CJOJmH sH nHtH_H2O2 \bhQe Ty2|u6O6\bhQ Ty2}vXDvuzOz\bhQS2/~da$$e&m^ #+D#$/0$$CJOJPJaJmH sH nHtH_HJOJN~e"XDYD^`OJPJVObVDU_N~agh. h & F2XD22YD28$@& h`O`RyfpeW[SVDX^XWD88`8CJOJmH sH nHtH_H`Ob`Se.s-a$$1$$$@&-DM OJPJaJKH2O122 \bhQe?z{|+R2u@OB@hQfN wNa$$mH sH nHtH_H0Ob0N~agh & F@&<Ob<Vhlf & F CJOJaJDOrDagel  & Fa$$ OJlOblckeVh' & FXD2YD2a$$ h CJOJPJmH sH nHtH_HOhQy>da$$8$7$1$5$3$&%+Dp#$/.0$*CJ0OJ5RH@mH sH nHtH_H\NONhQfN w_vPpeu :r a$$ :r OJPJnObnDU_hƋ< & Fa$$1$$@&-DM  hOJPJaJKHlOblckehh' & FXD2YD2a$$ h CJOJPJmH sH nHtH_H4O4 \bN'` z^hƋ2uJOJDU_N~e hXDYD OJPJaJh@hQ<eZ1lr %Y|O :^ W( H C1 k m s L= QO q F  V$_+ SNiYQb!@X&70,:(m!GYl@A:gD#}#$$}%e%w%"&x'&(5(si(pN)$* t*++TE+r,- .).5V.d.,j./!/90&Y2z2f3s?4`P4;25 6"?6dU718m89G99u":Oj:p:;u;x;<u<x<g>)W>N?S!@/@PBC)kD?EGuFG GG5J$KL/)L2nLQs=QCJQtQ#R &RVVR|!SLSK&T7T3UWWNFX@]X/YZXZ^ZdZ<[(\m\^lqaCbV.c\c3dBedeKeRe:flkf&h'hh#i.j^j;xj3{jNkukglzl` m"mXMx{3]]y)HwL3/GdfY 9l~)Z]5!QcN :Bbun!D#LdMt4airZ'jxxr< h+K7u{AK-C)M/fs8+Xc 2#>-)DaoL&{K:5:p:.........1 (  \   c *(( DT"\   c *(( LB"h  s 0((@ RQ#" `^  c ,(( BAH"bB   S *() v~ 10#" \B   S *() v~ 11"* 3 ?/Q8t WBt 8%8l # t 'C/"t $"t(+HTZ\hmo{#(,8;?KNS_dhtw{FFFFFFFFFFFFFF)+!ICSWXFLHStdNo1StdNo2DTStdNameWCRQFYFDSYSMSDfmBKQY StandardName _Toc310598115 _Toc310598151 _Toc310598269 _Toc310327186 _Toc310327254 _Toc310598168 _Toc310598286 _Toc310598612 _Toc310705442 _Toc310843421 _Toc310863991 _Toc340854247 _Toc341109955 _Toc341125785 OLE_LINK3 OLE_LINK4H\o?Sh{K_____333333333(( ,[n)Oex[######### 1 ( }ScHrCustom Popup 4675599 (B\!kh7h_Custom Popup 4675615 ( }Q[7h_Custom Popup 4675646%!o]D!-p_F!spaFCustom Popup 9960453 Custom Popup 99604530 ( \b( v!k( MR.( _*( DU_ ( Se.s( "}_( h"}_y(  Rd"}_yf( SbcHr( SbpSȉm( SbpS Custom Popup 9960500 Custom Popup 99605000( zh( N~aghZ( N~agh](  N~agh[( V~aghk( N~agh(ehN~ag(ehN~ag(eh N~ag(ehV~ag(ehN~ag( k ( Ry  N~ ( Ry%N~ ( Ry% N~ (  W[kSRyN~ 5(  W[kSRyN~ P(  peW[SRyN~ ( SRy N~ Custom Popup 9960562 Custom Popup 99605620( :yO( :yO}( :yO( agel( agel ( agelG( Vhld( Vhl$( Vhl( agel( Vh( hh( ceQh 6 iR$ iR$ d3Ps$$If:V 644l44l0&5& X 4 b r FvG 6 iR$ iR$ 6 iR$ iR$ 6 iR$ iR$ 6 iR$ iR$ G 6 iR$ iR$ 6 iR$ iR$ Gs 6 iR$ iR$ 6 iR$ iR$ 6 iR$ iR$ 6 iR$ iR$ fp|FP t,G 6 iR$ iR$ K 6 iR$ iR$ K 6 iR$ iR$ KG 6 iR$ iR$ 6 iR$ iR$ G 6 iR$ iR$ G@@G 6 iR$ iR$ XZ6 iR$WD` iR$ D J :r :r 6 iR$WD` iR$ 6 iR$WD` iR$ 96 iR$WD` iR$ 6 iR$ iR$ G 6 iR$ iR$ 30P. A!#7"R$n%2P1890P. A!#7"n$n%n2P180060P. A!#7"n$n%n2P180DStdNo2XXXXrDDTDStdName[Rir +s_uirf[I{~SvKmvDWCRQzDFYXXXXvDFMXXvDFDXXzDSYXXXXvDSMXXvDSDXXrDfm( SbpSȉm( SbpS ( ObTCSeN(  SmTCSeNObCustom Popup 3029835 Custom Popup 3029835,0( zh( N~aghG( N~aghH(  N~aghI( V~aghJ( N~aghK( ehN~ag( ehN~ag( eh N~ag( ehV~ag( ehN~ag( k ( Ry  N~ 8( Ry%N~ ( Ry% N~ (  W[kSRyN~ (  W[kSRyN~ 5(  peW[SRyN~ ( SRy N~ Custom Popup 3029867 Custom Popup 3029867-0( :yO( :yO( :yOo( :yOQ[,( agel( agel$( agel ( VhlB( VhlJ( VhlF( agel( Vhl&( Vlr( VhS( hh( ceQh